Emerging Technologies for Making Glycan-Defined Glycoproteins

Glycan analysis of therapeutic glycoproteins

Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell systems. The glycan moieties attached to the proteins can directly affect protein stability, bioactivity, and immunogenicity. Therefore, glycan variants of a glycoprotein product must be adequately analyzed and controlled to ensure product quality. However, the inherent complexity of protein glycosylation poses a...

Boiling Reduces Glycan Detection on Glycoproteins

Glycobiology is emerging as a primary field of interest for biological research. As such, it is crucial to develop and improve tools for the study of sugar-modified biomolecules. Many research endeavors in the field of protein glycobiology involve boiling of proteins in sample buffer for 5-10 minutes prior to gel electrophoresis and their further characterization. Here we examined the effect of...

Emerging technologies for learning

Emerging Technologies for Telemedicine

This paper focuses on new technologies that are practically useful for telemedicine. Three representative systems are introduced: a Digital Video Transport System (DVTS), an H.323 compatible videoconferencing system, and Vidyo. Based on some of our experiences, we highlight the advantages and disadvantages of each technology, and point out technologies that are especially targeted at doctors an...

Emerging technologies.

Hemodynamic monitoring in critically ill patients has been considered part of the standard of care in managing patients with shock and/or acute lung injury, but outcome benefit, particularly in pediatric patients, has been questioned. There is difficulty in validating the reliability of monitoring devices, especially since this validation requires comparison to the pulmonary artery catheter, wh...